Isotonic Solution Calculator – Check Solution Tonicity & Osmotic Balance



Reference Solution (Known)
mol/L (M)
e.g., 2 for NaCl
Target Solution (Unknown Molarity)
e.g., 1 for Glucose
Required Concentration
Target Molarity (C2)
0.300 M
Isotonic Condition Π1 = Π2
Formula Used C2 = (C1 · i1) / i2

About Isotonic Solution Calculator

Two solutions are called isotonic when they have the same effective osmotic concentration across a selectively permeable membrane. In simple terms, neither solution has a stronger tendency to draw water across the membrane than the other.

An Isotonic Solution Calculator can help compare the effective concentration of dissolved particles and determine whether a solution matches a chosen reference. The van’t Hoff factor can be important here because a substance that dissociates into several particles contributes more osmotically active particles than an equal molar concentration of a non-dissociating substance.

Understanding isotonicity is useful in chemistry, biology, pharmaceutical science, and studies of osmosis. The idea also explains why changing solute concentration can affect cells placed in different solutions.

When using a calculator, pay attention to the type of concentration being used and whether the solute produces additional particles in solution. For a simple ideal comparison, osmotic concentration is often considered through the product iC, where i is the van’t Hoff factor and C is concentration. Real solutions can behave differently from ideal solutions, especially at higher concentrations.